P. berghei kinesin-8B motor domain in AMPPNP state bound to tubulin dimer
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7z2b
Q-score: 0.367
Liu T, Shilliday F, Cook AD, Zeeshan M, Brady D, Tewari R, Sutherland CJ, Roberts AJ, Moores CA
Nat Commun (2022) 13 pp. 6988-6988 [ DOI: doi:10.1038/s41467-022-34710-x Pubmed: 36384964 ]
- Detyrosinated tubulin alpha-1b chain (47 kDa, Protein from pig)
- P. berghei kinesin-8b motor domain in amppnp state bound to tubulin dimer (Complex from Plasmodium berghei)
- Tubulin beta chain (47 kDa, Protein from pig)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Kinesin-8, putative (38 kDa, Protein from Plasmodium berghei)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
P. berghei kinesin-8B motor domain in no nucleotide state bound to tubulin dimer
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7z2a
Q-score: 0.253
Liu T, Shilliday F, Cook AD, Zeeshan M, Brady D, Tewari R, Sutherland CJ, Roberts AJ, Moores CA
Nat Commun (2022) 13 pp. 6988-6988 [ DOI: doi:10.1038/s41467-022-34710-x Pubmed: 36384964 ]
- Kinesin-8, putative (39 kDa, Protein from Plasmodium berghei)
- Tubulin beta chain (47 kDa, Protein from pig)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- P. berghei kinesin-8b motor domain in no nucleotide state bound to tubulin dimer (Complex from Plasmodium berghei)
- Magnesium ion (24 Da, Ligand)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Detyrosinated tubulin alpha-1b chain (47 kDa, Protein from Pig)
P. falciparum kinesin-8B motor domain in no nucleotide bound to tubulin dimer
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7z2c
Q-score: 0.296
Liu T, Shilliday F, Cook AD, Zeeshan M, Brady D, Tewari R, Sutherland CJ, Roberts AJ, Moores CA
Nat Commun (2022) 13 pp. 6988-6988 [ DOI: doi:10.1038/s41467-022-34710-x Pubmed: 36384964 ]
- Detyrosinated tubulin alpha-1b chain (47 kDa, Protein from pig)
- Tubulin beta chain (47 kDa, Protein from pig)
- Kinesin-like protein (39 kDa, Protein from Plasmodium falciparum)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- P. falciparum kinesin-8b motor domain in no nucleotide state bound to tubulin dimer (Complex from Plasmodium berghei)
Resolution: 4.4 Å
EM Method: Helical reconstruction
Fitted PDBs: 7nb8
Q-score: 0.268
Cook AD, Roberts AJ, Atherton J, Tewari R, Topf M, Moores CA
J Biol Chem (2021) 297 pp. 101063-101063 [ DOI: doi:10.1016/j.jbc.2021.101063 Pubmed: 34375637 ]
- Kinesin-5 (46 kDa, Protein from Plasmodium falciparum (isolate 3D7))
- Alpha/beta-tubulin (Complex from Sus scrofa)
- Kinesin-5 (Complex from Plasmodium falciparum (isolate 3D7))
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Pig)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- 14 protofilament microtubule of alpha/beta-tubulin dimers, with kinesin-5 motor domain bound 1:1 to tubulin dimers. (Complex)
- Tubulin alpha-1b chain (50 kDa, Protein from Pig)
Resolution: 4.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 7nba
Q-score: 0.268
Cook AD, Roberts AJ, Atherton J, Tewari R, Topf M, Moores CA
J Biol Chem (2021) 297 pp. 101063-101063 [ DOI: doi:10.1016/j.jbc.2021.101063 Pubmed: 34375637 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Pig)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Kinesin motor domain-containing protein,kinesin motor domain-containing protein (46 kDa, Protein from Plasmodium falciparum (isolate NF54))
- Tubulin alpha-1b chain (50 kDa, Protein from Pig)
- 14 protofilament microtubule complexed with plasmodium falciparum kinesin-5 motor domain bound to amppnp. asymmetric unit. (Complex from Plasmodium falciparum (isolate NF54))
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
Human kinesin-5 motor domain in the GSK-1 state bound to microtubules
Resolution: 3.8 Å
EM Method: Helical reconstruction
Fitted PDBs: 6ta3
Q-score: 0.215
Pena A, Sweeney A, Cook AD, Topf M, Moores CA
Structure (2020) 28 pp. 450-457.e5 [ DOI: doi:10.1016/j.str.2020.01.013 Pubmed: 32084356 ]
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Tubulin alpha-1b chain (48 kDa, Protein from Pig)
- Ternary complex of human kinesin 5 and microtubules (Complex)
- Kinesin-like protein kif11 (43 kDa, Protein from Homo sapiens)
- Kinesin-like protein kif11 (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 6-[4-(trifluoromethyl)phenyl]-3,4-dihydro-1~{h}-quinolin-2-one (291 Da, Ligand)
- Tubulin beta chain (48 kDa, Protein from Pig)
- Tubulin alpha-1b chain and tubulin beta chain (Complex from Sus scrofa)
Human kinesin-5 motor domain in the AMPPNP state bound to microtubules
Resolution: 6.1 Å
EM Method: Helical reconstruction
Fitted PDBs: 6ta4
Q-score: 0.156
Pena A, Sweeney A, Cook AD, Topf M, Moores CA
Structure (2020) 28 pp. 450-457.e5 [ DOI: doi:10.1016/j.str.2020.01.013 Pubmed: 32084356 ]
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Tubulin alpha-1b chain (48 kDa, Protein from Pig)
- Amppnp bound human eg5 motor domain in complex with alpha/beta tubulin (Complex from Homo sapiens)
- Kinesin-like protein kif11 (43 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (48 kDa, Protein from Pig)
- Amppnp bound human eg5 motor domain in complex with alpha/beta tubulin (Complex)
- Amppnp bound human eg5 motor domain in complex with alpha/beta tubulin (Complex from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
Microtubule-bound MKLP2 motor domain in the presence of ADP
Atherton J, Yu IM, Cook A, Muretta JM, Joseph A, Major J, Sourigues Y, Clause J, Topf M, Rosenfeld SS, Houdusse A, Moores CA
eLife (2017) 6 [ Pubmed: 28826477 DOI: doi:10.7554/eLife.27793 ]
- Complex of 14pf microtubule with bound mklp2 motor domain in the presence of adp (Complex)
- Taxol (853 Da, Ligand)
- Tubulin beta-2b chain (49 kDa, Protein from Bos taurus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tubulin alpha and beta chains (Complex from Bos taurus)
- Tubulin alpha chain (50 kDa, Protein from Bos taurus)
- Kinesin-like protein kif20a (56 kDa, Protein from Mus musculus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Kinesin-like protein kif20a (Complex from Mus musculus)
Microtubule-bound MKLP2 motor domain in the with no nucleotide
Atherton J, Yu IM, Cook A, Muretta JM, Joseph A, Major J, Sourigues Y, Clause J, Topf M, Rosenfeld SS, Houdusse A, Moores CA
eLife (2017) 6 [ DOI: doi:10.7554/eLife.27793 Pubmed: 28826477 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta-2b chain (49 kDa, Protein from Bovine)
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha and beta chains (Complex from Bos taurus)
- Complex of 13pf taxol-gdp microtubule with bound mklp2 motor domain with no-nucleotide (Complex)
- Taxol (853 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin alpha chain (50 kDa, Protein from Bovine)
- Kinesin-like protein kif20a (Complex from Mus musculus)
- Kinesin-like protein kif20a (56 kDa, Protein from Mus musculus)
Microtubule-bound MKLP2 motor domain in the presence of ADP.AlFx
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 5nd4
Q-score: 0.148
Atherton J, Yu IM, Cook A, Muretta JM, Joseph A, Major J, Sourigues Y, Clause J, Topf M, Rosenfeld SS, Houdusse A, Moores CA
eLife (2017) 6 [ Pubmed: 28826477 DOI: doi:10.7554/eLife.27793 ]
- Taxol (853 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tubulin alpha and beta chains (Complex from Bos taurus)
- Complex of 14pf microtubule with bound mklp2 motor domain in the presence of adp.alfx (Complex)
- Kinesin-like protein kif20a (56 kDa, Protein from Mus musculus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta-2b chain (47 kDa, Protein from Bos taurus)
- Tubulin alpha chain (45 kDa, Protein from Bos taurus)
- Magnesium ion (24 Da, Ligand)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Kinesin-like protein kif20a (Complex from Mus musculus)